Apejuwe alaye
Awọn ohun elo idanwo iyara ti ọlọjẹ parvovirus antijeni nlo ilana ti ọna ipanu ipanu apakokoro meji lati ṣe iwari antijeni ireke parvovirus ni didara ni awọn idọti aja.Aṣa goolu paravovirus antibody 1 ni a lo bi ami atọka, ati agbegbe wiwa (T) ati agbegbe iṣakoso (C) lori awo nitrocellulose ni a bo pẹlu aja aja parvovirus antibody 2 ati agutan egboogi-adie, lẹsẹsẹ.Ni akoko wiwa, ayẹwo jẹ chromatographic labẹ awọn ipa capillary.Ti ayẹwo idanwo naa ba ni antijeni aja parvovirus, goolu boṣewa antibody 1 ṣe eka antigen-antibody pẹlu aja aja parvovirus, ati pe o daapọ pẹlu paravovirus apakokoro 2 ti o wa titi ni agbegbe wiwa lakoko chromatography lati ṣe agbekalẹ “antibody 1-antigen-antibody 2″ sandwich , Abajade ni ẹgbẹ-awọ-awọ-pupa ni agbegbe wiwa (T);Ni idakeji, ko si awọn ẹgbẹ pupa-pupa ti o han ni agbegbe wiwa (T);Laibikita wiwa tabi isansa ti antigen parvovirus ireke ninu apẹẹrẹ, eka IgY ti adie boṣewa goolu yoo tẹsiwaju lati wa ni siwa si oke si agbegbe iṣakoso (C), ati pe ẹgbẹ pupa-pupa yoo han.Ẹgbẹ pupa-pupa ti a gbekalẹ ni agbegbe iṣakoso (C) jẹ boṣewa fun ṣiṣe idajọ boya ilana chromatography jẹ deede, ati tun ṣiṣẹ bi boṣewa iṣakoso inu fun awọn reagents.
Adani Awọn akoonu
